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KMID : 0364219880310040300
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Korean Journal of Zoology
1988 Volume.31 No. 4 p.300 ~ p.308
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Studies on the Generation and Application of Monoclonal Antibodies against Tumor Marker Antigen 1. Production and Characterization of Monoclonal Antibodies against Placental Alkaline Phosphatase
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Kim Han-Do
Kang Ho-Sung
Kim Hyun-Mee
Kang Shin-Sung
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Abstract
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Human placental alkaline phosphatase (PLAP), one of the oncofetal antigen was purified from placentas through the procedures including butanol extraction, concanavalin A-Sephar-ose, DEAE-cellulose and Sephadex G-200 gel chromatography. Monoclonal antibodies (fibs) against human PMP were produced by hybridizing SP 210-Ag 14 mouse myeloma cells with spleen ceils of Balblc mice immunized with PLAP. Six stable monoclones uvere obtained by cloning tuvice in serial dilutions, and the monoclonal speclfidty of these MAbs was confirmed by biochemical and immunonogical criteria.
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KEYWORD
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Tumor marker antigen , Alkalinephosphatase , Monocloanl antibodies
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